There are individual differences in the animals, not only in how they will respond to your immunogen, but also in what is already present their system. Screening pre-immune samples from a number of individuals is a good idea in order to select one which will give the lowest background in your application.
Typically you will have to test 3-5 individuals per intended immunisation. In most cases this number suffices, but there have been cases where 10 animals needed to be tested before finding a suitable candidate.
However, if you are planning on using material that has been antigen specific affinity purified – especially if the antigen is a synthetic peptide - then it is questionable if the screening is meaningful.
Innovagen was founded in 1992 and over the years we have produced thousands of custom antibodies, in various hosts (rabbit, chicken, goat, etc) against most types of antigens. Our knowledge and dedication to quality in all aspects of the production stems from our extensive experience in this area. This will be of great benefit for you when you turn to us for your antibody requirements.
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